Characterisation of rhamnogalacturonan-II (RG-II) preparations by gel electrophoresis.

(a) Non-volatile solutes of red wine were dissolved at 1% (w/v) in water and electrophoresed before (i) and after (ii) 24 h of dialysis. (b) Electrophoresis of various volumes of a RG-II-rich preparation from Arabidopsis cultures grown in standard medium (containing 100 μm H3BO3). The 1-μl sample contained about 0.2 μg RG-II. (c) Rosa cells were grown with 0, 0.3 or 3.3 μm H3BO3 for various periods, the medium being renewed fortnightly. Rhamnogalacturonan-II from their cell walls was electrophoresed.

(Fig. from: Chormova D, Messenger DJ, Fry SC. Boron bridging of rhamnogalacturonan-II, monitored by gel electrophoresis, occurs during polysaccharide synthesis and secretion, but not post-secretion. Plant J 77:534–546, 2013)